Workshop: Day 4

It’s a Wrap!!

As we didn’t have much work yesterday . Hence we could seen how exactly Silver Staining works.

Analysis is the most important aspect, especially if you are studying Bioanalytical Sciences, Biotechnology, etc. Any lab technician without much knowledge can do the steps but it takes only a good analytical mind to do the analysis well.

What is Silver Staining?

Silver staining is used to detect protein after electrophoretic separation on polyacrylamide gels. The sequential phases of silver staining are protein fixation then sensitization, then silver impregnation and finally image development.

Based on the size/interaction of protein with the gel the separation happens. From which we can conclude that thicker the band more is the protein content.

So why Silver Staining over CBB ?

Classical Coomassie Brillant Blue  staining can usually detect a 50 ng protein band ; silver staining increases the sensitivity typically 50 times.

Many variables can influence the color intensity and every protein has its own staining characteristics; clean glassware, pure reagents and water of highest purity are the key points to successful staining.

This workshop taught us many things in four day . I realized practical is much more from what we study in theory. Just reading up stuff will not help us to understand concepts. Hands on training have changed our outlook regarding the subject. Dealing with microlitres of solutions was beyond imagination. But we have now realized that such minute quantities makeup to the fundamentals of our lives.

Concluding I would sum it up as:

  1. It was a great experience.
  2. Hands-on training was the best thing we could have done.
  3. Patient faculty.
  4. Good exposure.
  5. Trouble shooting was learnt too!

This learning experience was very enjoyable, exciting and more informative. We would like to Thanks our HOD, Mr. Sachin Palekar  for arranging such a wonderful workshop. Special Thanks to Mrs. Anjali Apte Deshpande Ma’am for coming and teaching us various techniques like Plasmid Extraction, PCR, SDS-PAGE, etc.  Also  Amrutha Ma’am and Narendra Sir for helping us and solving our doubts.

Looking forward to attend more such workshop!!  🙂 🙂

Draft by: Bhavana Kaloni

                  Asawari Malawi

                 Bhagyashree Rajput

 

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